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Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. Galactolipase contributes to milk fat hydrolysis. It hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase. Galactolipase also hydrolyzes both phospholipids and galactolipids. It acts preferentially on monoglycerides, phospholipids and galactolipids.