CDH1 monoclonal antibody (M01), clone 3F4
产品名称： CDH1 monoclonal antibody (M01), clone 3F4
英文名称： CDH1 monoclonal antibody (M01), clone 3F4
产品编号： H00000999-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant CDH1.
- Immunogen:
- CDH1 (NP_004351, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- KGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDV
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Development of an AlphaLISA assay to quantify serum core-fucosylated E-cadherin as a metastatic lung adenocarcinoma biomarker.
Wen CL, Chen KY, Chen CT, Chuang JG, Yang PC, Chow LP.J Proteomics. 2012 May 23.
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody (M01), clone 3F4.
Lane 1: CDH1 transfected lysate(97.5 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to CDH1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.375 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged CDH1 is 0.1 ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between EGFR and CDH1 HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-CDH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 999
- GeneBank Accession#:
- NM_004360
- Protein Accession#:
- NP_004351
- Gene Name:
- CDH1
- Gene Alias:
- Arc-1,CD324,CDHE,ECAD,LCAM,UVO
- Gene Description:
- cadherin 1, type 1, E-cadherin (epithelial)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq
- Other Designations:
- cadherin 1, E-cadherin (epithelial),cadherin 1, type 1,calcium-dependent adhesion protein, epithelial,cell-CAM 120/80,uvomorulin
- Related Disease
- Adenocarcinoma
- Asthma
- Asthma
- Breast cancer
- Breast Neoplasms
- Bronchiolitis, Viral
- Carcinoma
- Carcinoma, Ductal, Breast
- Carcinoma, Hepatocellular
- Carcinoma, Large Cell
- Carcinoma, Lobular
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Renal Cell
- Carcinoma, Squamous Cell
- Carcinoma, Transitional Cell
- Cleft Lip
- Cleft Palate
- Colitis, Ulcerative
- Colorectal Neoplasms